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A quantitative estimation of the energetic cost of brown ring disease in the Manila clam using Dynamic Energy Budget theory ArchiMer
Flye Sainte Marie, Jonathan; Jean, Fred; Paillard, Christine; Kooijman, Sebastiaan.
Brown ring disease (BRD) in the Manila clam, Ruditapes philippinarum, is a bacterial disease caused by the pathogen Vibrio tapetis. This disease induces the formation of a characteristic brown conchiolin deposit on the inner shell and is associated with a decrease in condition index indicating that the development of the disease affects the energy balance of the clam. A previous study showed that the energy budget of the host was affected by a decrease in filtration activity, and hypothesized that a second way to degrade the energy balance was the increase in maintenance costs associated to the cost of immune response and lesion repair. This paper focusses on this second way of degradation of the energy balance. A starvation experiment confirmed that the...
Tipo: Text Palavras-chave: Maintenance Cost; Dynamic Energy Budget Theory; Energetic Cost; Energy Budget; Brown Ring Disease; Manila Clam.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6787.pdf
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Impact of Brown Ring Disease on the energy budget of the Manila clam Ruditapes philippinarum ArchiMer
Flye Sainte Marie, Jonathan; Pouvreau, Stephane; Paillard, Christine; Jean, Fred.
Brown Ring Disease (BRD) is a bacterial disease caused by the pathogen, Vibrio tapetis. The disease induces formation of a brown deposit on inner shell of the Manila clam, Ruditapes philippinarum. Development of this disease is correlated with a decrease in the condition index of infected clams. Experiments were conduced in order to assess the effect of the development of BRD on two parameters affecting the energy balance of the clams: the clearance and the respiration rates. Experiments were performed in a physiological measurement system that allowed simultaneous measures of clearance and respiration rates. During both acclimation and measurements clams were fed with cultured T-iso and temperature was close to seasonal field temperature (10 degrees C....
Tipo: Text Palavras-chave: Ruditapes philippinarum; Respiration rate; Filtration; Energy budget; Clearance rate; Brown Ring Disease.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3013.pdf
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Implication of the Type IV Secretion System in the Pathogenicity of Vibrio tapetis, the Etiological Agent of Brown Ring Disease Affecting the Manila Clam Ruditapes philippinarum ArchiMer
Rahmani, Alexandra; Delavat, François; Lambert, Christophe; Le Goic, Nelly; Dabas, Eric; Paillard, Christine; Pichereau, Vianney.
Vibrio tapetis is a Gram-negative bacterium that causes infections of mollusk bivalves and fish. The Brown Ring Disease (BRD) is an infection caused by V. tapetis that primarily affects the Manila clam Ruditapes philippinarum. Recent studies have shown that a type IV secretion system (T4SS) gene cluster is exclusively found in strains of V. tapetis pathogenic to clams. However, whether the T4SS is implicated or not during the infection process remains unknown. The aim of this study was to create and characterize a V. tapetis T4SS null mutant, obtained by a near-complete deletion of the virB4 gene, in order to determine the role of T4SS in the development of BRD. This study demonstrated that the T4SS is neither responsible for the loss of hemocyte adhesion...
Tipo: Text Palavras-chave: Brown Ring Disease; Vibrio tapetis; Ruditapes philippinarum; Hemocytes; Type four secretion system; Rounding phenotype; Colonization; Mutagenesis.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00692/80383/83507.pdf
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Vibrio tapetis, the Causative Agent of Brown Ring Disease, Forms Biofilms with Spherical Components ArchiMer
Rodrigues, Sophie; Paillard, Christine; Le Pennec, Gael; Dufour, Alain; Bazire, Alexis.
Vibrio tapetis is a marine bacterium causing Brown Ring Disease (BRD) in the Manila clam Ruditapes philippinarum. V. tapetis biofilm formation remains unexplored depite the fact that it might be linked to pathogenicity. Our objectives were to characterize the in vitro biofilm formation of V tapetis and evaluate the effects of culture conditions. Biofilm structure and its matrix composition were examined by confocal laser scanning microscopy and scanning electron microscopy. V tapetis was able to form biofilms on a glass substratum within 24 h. Polysaccharides and extracellular DNA of the biofilm matrixes were differently distributed depending on the V tapetis strains. Spherical components of about 1-2 mu m diameter were found at the biofilm surface. They...
Tipo: Text Palavras-chave: Vibrio tapetis; Biofilm; Brown Ring Disease; Ruditapes philippinarum; Spherical components; Temperature effect; Salinity effect.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00307/41783/42796.pdf
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